Is this tool private?

Yes. All processing happens locally in your browser. No data is uploaded.

What extras does this tool have?

Extras: (1) Core function, (2) Batch mode, (3) Copy results, (4) Download, (5) History, (6) CSV export, (7) Custom options, (8) Validation, (9) Warnings, (10) Stats, (11) Multi-format, (12) Configurable, (13) Preview.

Calculators Runs in your browser

Enzyme Activity Calculator

Calculate enzyme activity (U/mL, specific activity). 100% private. 100% private.

100% Private Works Offline Instant

ΔA (absorbance change)

Δt (min)

Total assay volume (mL)

Extinction coefficient ε (M⁻¹·cm⁻¹)

Path length l (cm)

Sample volume v (mL)

Enzyme concentration (mg/mL)

Activity result

U/mL

0.0002 U/mL

0.2 U/L · katal: 3.334e-12

Specific activity: 0.0002 U/mg

Activity = (ΔA/Δt × V) / (ε × l × v) = (0.1000 × 1) / (6220 × 1 × 0.1) = 0.0002 U/mL

Turnover number kcat

Vmax (U/mL)[E] (M)

kcat = 1666.6667 s⁻¹

Michaelis-Menten

VmaxKm[S]

v = 50

Lineweaver-Burk (1/[S] vs 1/v)

1/[S]=0.5  1/v=0.06
1/[S]=0.2  1/v=0.03
1/[S]=0.1  1/v=0.02
1/[S]=0.05  1/v=0.015
1/[S]=0.02  1/v=0.012

Batch (ΔA,Δt,V,ε,l,v per line)

0.0002 U/mL

0.0003 U/mL

Privacy: all calculations run locally in your browser.

Calculate enzyme activity (U/mL, specific activity). 100% private. 100% private. Everything runs locally in your browser — your data never leaves your device.

How to use

Enter your input in the tool above.
Adjust any options to your preference.
Use the Copy or Download buttons to save the result.
Everything happens locally — your data never leaves your browser.

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